Anti-Interleukin-10 (IL-10), clone 5J48

Interleukin-10 (IL-10), originally known as Cytokine Synthesis Inhibitory Factor (CSIF), shares over 80% sequence homology with the Epstein-Barr Virus protein BCRFI. The reported biological activities of IL-10, which may be interrelated, include inhibition of macrophage-mediated cytokine synthesis, suppression of the delayed-type hypersensitivity response, and stimulation of the Th2 cell response which results in elevated antibody production. Human IL-10 is active on murine cells. Murine IL-10 is inactive on human cells. Recombinant human IL-10 is an 18.6kD protein with 161 amino acid residues. Application: Suitable for use in ELISA as a capture antibody. Other applications have not been tested. Recommended Dilutions: ELISA: 1-5ug/ml, Coat each well with 100ul., Optimal dilutions to be determined by the researcher. Recommended Pair: Suitable for use as the capture or solid phase antibody. , Matched to I8432-16 as the detection or conjugate (label) antibody. Hybridoma: BALB/c splenocytes were fused with NSO mouse myeloma cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.