Anti-Interleukin-10 (IL-10) (azide-free), clone 4H189

Applications:, ELISA Detection: Useful as a detection antibody in a sandwich ELISA for quantifying mouse IL-10 protein levels. Biotinylated antibody should be paired with purified antibody (Cat. No. I8432-18A) as the capture antibody, with purified recombinant mouse IL-10 as the standard., Immunofluorescence/Flow Cytometry: Useful for intracytoplasmic staining and flow cytometric analysis to identify and enumerate IL-10-positive cells within mixed cell populations. Recombinant mouse IL-10, unlabeled (Cat. No. I8432-24B), or unlabeled or biotinylated Rat IgG2b should be used as a specificity control., Neutralization: The LE/AF form can be used for neutralizing mouse IL-10 bioactivity. A suitable LE/AF Rat IgG2b should be used as an isotype control. Recommended Dilution: Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20°C or colder. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.