Anti-Interleukin 1 Receptor Type I (Interleukin Receptor 1, IL-1R-1, IL1R1, IL-1RT1, IL1RT1, Interle

Two distinct receptors bind the pleiotropic cytokines IL-1a and IL-1b. The IL-1 Receptor I (IL-1 RI) is an 80kD transmembrane protein expressed predominantly in T cells, fibroblasts, and endothelial cells. IL-1 Receptor II is a 68kD protein that is found on B lymphocytes, neutrophils, monocytes, large granular leukocytes, and endothelial cells. Both receptors are members of the immunoglobulin superfamily and show ~28% sequence identity in their extracellular domains. The two receptors do not heterodimerize into a receptor complex. Soluble recombinant IL-1 Receptor I is a potent antagonist of IL-1 action. Applications: Suitable for use in FLISA, Western Blot and Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 10ul/10e6 HUVEC human umbilical vein endothelial cells, Optimal dilutions to be determined by the researcher. Absorption: 620-650nm , Emission: 660-670nm, Storage and Stability: May be stored at 4°C before opening. DO NOT FREEZE! Stable at 4°C as an undiluted liquid. Stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap. Further dilutions can be made in assay buffer. APC is light sensitive.