Anti-Interleukin-1 Receptor Accessory Protein (IL1RacP, IL-1 Receptor AcP)

The pro-inflammatory cytokine IL-1 induced cellular response requires two subunits of its receptor, IL-1 receptor I (IL-1RI) and IL-1 receptor accessory protein (IL-1RAcP). IL-1RAcP forms a complex with IL-1RI in response to IL-1 treatment. The IL-1 receptor-associated kinase (IRAK), which mediates activation of NF-kB inducing kinae (NIK) and of NF-kB, recruits to the IL-1R complex through IL-1RAcP. IL-1 activation of stress-activated protein kinase and of acid sphingomyelinase also requires IL-1RAcP. Like IL-1RI, IL-1RAcP subunit is essential for IL-1 mediated cellular response. IL-1RAcP is expressed in many tissues. Applications: Suitable for use in ELISA and Western Blot. Other applications have not been tested. , Recommended Dilutions: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: HeLa cell lysates, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.