Anti-Interferon Regulatory Factor 5 (IRF5, interferon regulatory factor 5)

IRF5 (interferon regulatory factor 5) is a nuclear protein and member of the IRF family, which acts as a key regulator of interferon (IFN) gene transcription. IRF5 is expressed by B cells and dendritic cells, is inducible by type I interferon (IFN) in most lymphoid cells, and has the ability to induce a wide range of chemokines involved in T-lymphocyte recruitment, including RANTES, MCP-1 and IP-10. Genetic variations in IRF5 are associated with susceptibility to rheumatoid arthritis (RA), inflammatory bowel disease type 14 (IBD14) and systemic lupus erythematosus type 10 (SLEB10). , Domain Information: interferon regulatory factor, Gene Ontology Terms: RNA polymerase III transcription factor activity, transcription factor activity, cell growth and/or maintenance, regulation of transcription, DNA-dependent, nucleus , Application(s): Suitable for use in ELISA, Western Blot. Other applications not tested., Western blot: Approx 55-60kD band seen in A549 lysate (predicted MW 58kD). Recommended for use at 1-3ug/ml. Staining (2ug/ml) of A549 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence. Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Peptide Blocking: Corresponding peptide is available for Peptide Blocking studies. See I7662-89A-P. Antibody is typically 0.5mg/ml and peptide is supplied as a 100ul pellet. When peptide is reconstituted in 200ul water, the concentration would also be 0.5mg/ml. To start, the best ratio would be 1:1 (molar excess of peptide relative to antibody when identical volumes are mixed). Mix equal volumes of peptide and antibody at the required dilution and leave at ambient temperature. It is best is to have two identical blots to be incubated with equal amount of antibody, but one with the antibody pre-adsorbed to the peptide for 20min. Then incubate and develop the two blots in parallel.