Anti-Interferon gamma (IFNg), clone 6A568

Possible applications include the purification of human gamma Interferon and in vitro neutralization. In general, monoclonal antibodies to gamma Interferon are able to inhibit Schwartzman reactions and in the murine system appear to protect NZB mice against spontaneous development of autoimmune disease. Applications: Can be used for immunoassays, Immunohistology on frozen sections and Immunoprecipitation. Furthermore the antibody is useful for Neutralization of the biological activity of Interferon gamma. Recommended Dilution: Immunohistology: 1:10, For neutralization of biological activity in vitro dilutions have to be made according to the amount of IFN-gamma to be inactivated., Optimal dilutions to be determined by the researcher. Unit Definition: One neutralizing unit of anti-human gamma interferon is defined as the amount of antibody sufficient for neutralizing one unit human gamma interferon (ref.: NIH standard Gg23-901-530). Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.