Anti-Interferon gamma (IFNg), Biotin conjugated, clone 2B2.115

Interferon-gamma (IFN-gamma) is a pro-inflammatory cytokine that is central in host resistance to infection. It is mainly produced by natural killer cells and CD4+ and CD8+ T cells, its receptors are found on nearly all cells, where it activates diverse responses that enable potential host cells to prevent invasive infection by bacteria, parasites and viruses. Takayanagi et al. (2000) demonstrated that IFN-gamma strongly suppresses osteoclastogenesis by interfering with the RANKL (602642)-RANK (603499) signaling pathway. Tsubota et al. (1999) reported that this upregulation in Sjogren syndrome patients may be controlled by interferon-gamma through the activation of transcription factor NFKB. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilutions: ELISA (Detection): 0.1-1ug/ml. Dilutions should be made in a buffer appropriate for use in ELISA, such as PBS supplemented with 0.5% BSA, 0.1% Tween-20. , Optimal dilutions to be determined by the researcher. Hybridoma: Balb/c (Charles River) / Sp2/O Ag14, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.