Anti-Integrin, beta5 (Biotin)

Integrin beta5 is a 100-110kD type I transmembrane glycoprotein that forms a non-disulfide-linked heterodimer with Integrin alphaV. Integrin alphaVbeta5 is a receptor for vitronectin that is expressed on a wide variety of cell types including keratinocytes, fibroblasts, adhesive monocytes, and select endothelium. The 696 amino acid residue Integrin beta5 extracellular region contains a vWF-A domain and a cysteine-rich tandem repeat region. Human Integrin beta5 shares 91% and 89% amino acid sequence homology with mouse and canine Integrin beta5, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect human Integrin beta5. The detection limit for rhIntegrin beta5 is approximately 2 ng/lane and 10 ng/lane under non-reducing and reducing conditions, respectively. In this format, this antibody shows less than 5% cross-reactivity with Integrin beta1, -beta2, -beta3, rmIntegrin beta4, -beta6, and -beta7. , Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile TBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.