Anti-Integrin beta1, active, clone 2Q839

The beta1 or VLA integrin subgroup contains at least 10 members, each with a distinct alpha subunit non-convalently associated with the common b1 subunit. Functional activation of integrins can be experimentally induced by addition of Mg2+ or Mn2+, or by using one of several stimulatory antibodies that are available. Intracellular signals generated as a result of cell activation also modulate the confirmation and affinity of integrins in cellular systems. Antibodies which have the ability to discriminate between different states of integrin activation are exceptionally useful for studies on the regulation of activation. This antibody recognizes epitopes in the 355-425 region of the common b1 subunit of VLA integrins, whose expression parallels the ligand binding activity of these adhesion receptors induced by various extracellular and intracellular stimuli. Applications: Suitable for use in ELISA, Western Blot, FACS and Immunohistochemistry. Other applications not tested. Recommended Dilutions: FACS: 2.5ug/5x10e5 cells , Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.