Anti-Integrin alpha8 (Biotin)

Integrin alpha 8 is a 170-200kD member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin b1. Alpha8beta1 promotes both cell adhesion and survival and is known to bind to fibronectin, the latency-associated peptide in latent TGFb1 and nephronectin. Mouse Integrin b8 is a 1025 aa type I transmembrane glycoprotein. It contains a 969aa extracellular region (aa38-1006) and a 29aa cytoplasmic domain. One isoform may exist that shows a truncation after Ala675 of the precursor. In the ECD, mouse Integrin a8 shares 96% and 90% aa sequence identity with rat and human Integrin a8 protein, respectively. Applications: Suitable for use in Flow Cytometry, Western Blot and Immunocytochemistry. Other applications not tested. Recommended Dilutions: Flow cytometry: 2.5ug/10e6 cells. Tested on 4T1 mouse breast cancer cell line., Western Blot: 0.1ug/ml using recombinant mouse Integrin a8., Immunocytochemistry: 5-15ug/ml using Immersion fixed 4T1 mouse breast cancer cell line, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized and reconstituted products are stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.