Anti-Insulin-Like Growth Factor I Receptor (IGF1R, IGFIR, CD221, Clone 1900 Unknown Protein, JTK13,

Insulin-Like Growth Factor I Receptor (IGF-I Receptor) , a 155kD receptor tyrosine kinase, also known as CD221. CD221 is composed of two extracellular alpha-subunits and two transmembrane beta-subunits. CD221 is expressed in a range of tissues, including kidney, liver, placenta, mammary gland, brain, ovary and skin. The ligands for CD221 include IGF-I and IGF-II, which bind to CD221 and activate tyrosine kinase activity, resulting in phosphorylation of several intracellular signalling proteins. Clone 1H7 is reported to partically block binding of IGF-I and IGF-II to CD221 (1). Applications: Suitable for use in Flow Cytometry, Functional Assays, Immunohistochemistry (paraffin), and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:10, 10ul labels 10e6 cells in 100ul, Immunohistochemistry (paraffin): 1:10-1:40, Optimal dilutions to be determined by the researcher. Hybridoma: Sp2/0 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.