Anti-Insulin-Like Growth Factor Binding Protein-Related Protein 10 (IGFBP-rP10)

IGFBP-rP10, also known as Kazal-type serine protease inhibitor domain-containing protein 1 and bone and odontoblast-expressed protein 1, belongs to the IFGBP superfamily. It is a 276 amino acid (aa) residue, 36 kD secreted protein that contains an N-terminal IGFBP domain, a Kazal-type serine protease inhibitor region, a C2-type Ig-like domain and a C-terminal poly-glu segment. An alternate variant with a 16 aa substitution for the C-terminal 33 residues has also been reported. IGFBP-rP10 is expressed in multiple tissues including osteoblasts/odontoblasts and is up-regulated during osteoblast differentiation and bone regeneration. Mouse mature IGFBP-rP10 shares 96% and 87% aa sequence identity with rat and human IGFBP-rP10, respectively. Applications: Western Blot: This antibody can be used at 0.1-0.2ug/ml with the appropriate secondary reagents to detect mouse IGFBP-rP10. The detection limit for rmIGFBP-rP10 is approximately 1 ng/lane under non-reducing and reducing conditions. , Direct ELISA: This antibody can be used at 0.5-1.0ug/ml with the appropriate secondary reagents to detect mouse IGFBP-rP10. The detection limit for rmIGFBP-rP10 is approximately 0.5 ng/well. , Optimal dilutions should be determined by each laboratory for each application. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.