Anti-Insulin-Like Growth Factor Binding Protein-1 (IGFBP1)

IGFBP-1 (Insulin-like Growth Factor Binding Protein-1) is produced by the liver and regulated by glucocorticoids and insulin at the level of gene transcription. The N-terminal and C-terminal regions are highly homologous among rat, human and bovine sequences. IGFBP-1 inhibits IGF binding to cell surface receptors and thereby inhibits IGF-mediated mitogenic and cell metabolic actions. Applications: Suitable for use in Western Blot, Immunocytochemistry and Immunoprecipitation. Other applications have not been tested. Recommended Dilutions: Western Blot: 1:1000 detects 10ng SDS-denatured human IGFBP-1., Immunoprecipitation: 1:500 precipitates >90% of 10ng IGFBP-1. , Optimal dilutions to be determined by the researcher. Recommended Secondary Antibodies: I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb, I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb, I1904-46Q: IgG, H&L (HRP) Pab Gt xRb, Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.