Anti-Immunoglobulin-Like Transcript 7 (ILT7, CD85k)

LILRA4, also known as ILT7 and CD85g, is a 499 amino acid (aa) type I transmembrane glycoprotein that contains four Ig­like domains in its extracellular region. LILRA4 is selectively expressed on plasmacytoid dendritic cells (pDC). It binds to BST2/CD317 on bone marrow stromal cells and associates with the gamma subunit of Fc epsilon RI. This receptor complex transmits signals that inhibit the TLR­induced production of type I interferon. The LILRA4­mediated interactions between pDC and tumor cells similarly inhibit the production of proinflammatory cytokines and contribute to reduced anti­tumor immune responses. Alternate splicing of human LILRA4 generates an isoform that lacks the N­terminal 43 aa of the extracellular domain. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 2.5ug/10e6 cells. Detection of ILT7/CD85g in Human PBMCs., Optimal dilutions to be determined by the researcher. Hybridoma: Myeloma cells with B cells from Balb/c mice. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.