Anti-IL2RG (Cytokine Receptor Common Subunit gamma, Interleukin-2 Receptor Subunit gamma, IL-2 Recep

The g chain of the high affinity functional human IL-2 receptor complex belongs to the hematopoietin receptor family. IL-2 Rg is a 369aa protein consisting of a 22aa signal sequence, a 232aa extracellular domain, a 29aa transmembrane domain and an 86aa cytoplasmic domain. Although IL-2 Rg by itself does not bind IL-2 with any appreciable affinity, it is required for IL-2 receptor signaling. Besides IL-2, the g chain has been shown to be a component of the functional receptor complexes for IL-4, IL-7, IL-9 and IL-15. IL-2 Rg has been designated the common g chain (gc). The site of molecular defects in X-linked SCID (severe combined immunodeficiency) has been mapped to the IL-2 Rg gene. Applications: Suitable for use in Flow Cytometry, Neutralization, Direct ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 2.5ug labels 10e6 using human peripheral blood lymphocytes, Neutralization: Measured by its ability to neutralize IL­2­-induced proliferation in the MO7e human megakaryocytic leukemic cell line (ref 3). The Neutralization Dose (ND50) is typically 0.08-0.4ug/ml in the presence of 200ng/ml of recombinatn human IL-2, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months after receipt at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.