Anti-IKKalpha (IkappaB Kinase alpha, IKKa, IKK1)

NFk B comprises a family of cellular transcription factors that are involved in the inducible expression of a variety of cellular genes that regulate the inflammatory response and control of cell death. In the cytoplasm NFk B is negatively modulated by the inhibitory proteins Ik B. In turn Ik B is phosphorylated by a cellular kinase complex called IKK. IKK is a heterodimer composed of two kinases: IKK-a and IKK-b that phosphorylate Ik B leading to its degradation and the resulting translocation of NFk B to the nucleus. IKK kinase activity is modulated negatively by pharmaceutical agents such as aspirin and positively by various cellular components such as TNF-a , endotoxins and overexpression of cellular kinases like MEKK1. Aspirin appears to have its effect by inhibiting the binding of ATP to IKK. Applications: Suitable for use in Immunoprecipitation, Western Blot and ELISA. Other applications not tested. Recommended Dilution: Western Blot: 1:1000. Reactive against IKKa followed by reaction with I1904-46Q. Detects a band at ~ 85kD. , Optimal dilutions to be determined by the researcher., ELISA: 1:5000-1:25,000, Optimal dilutions to be determined by the researcher. Control peptide: I3000-04B , Storage and Stability: , May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.