Anti-Hsp27 (Heat Shock Protein 27) (BSA & azide free), clone 0.N.321

HSP27 is identical to the estrogen-induced p29 and 24K protein. About 50% of breast carcinomas are positive for HSP27 especially those that are also positive for estrogen and/or progesterone receptor. HSP27 has also been implicated in drug resistance in cancer cells. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications have not been tested. Recommended Dilutions: Western Blot: 1-2ug/ml for 2 hours at RT., Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes., Optimal dilutions to be determined by the researcher. Positive Control: BT474, MDA-MB-453 cells, and breast carcinoma. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.