Anti-HMGA1B (High mobility group A1, Isoform B, HMG-Y)

HMGA1B (High mobility group A1, isoform B, also HMG-Y) is a 10-11kD member of the HMGA family of proteins. It is ubiquitously expressed, and occurs principally in tumor and embryonic tissue. HMGA1B is a nonhistone architectural protein that binds to AT-rich DNA sequences. It participates in both gene repression and activation by changing DNA conformation, and is known to regulate miRNA as well. Human HMGA1B is 96aa in length and contains three A-T hook DNA-binding domains (aa21-31, 42-52, 67-78). It is constitutively phosphorylated, and may undergo acetylation. There are three potential splice variants. All three show the same 11aa insertion after Pro34. In addition, one form shows an additional 56aa substitution for aa55-96, while a second shows a 14aa substitution for aa80-96. Full-length human HMGA1B shares 97% aa identity with mouse HMGA1B. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C for short-term only. Reconstitute with sterile 40-50% glycerol, aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.