Anti-HMG4 (High Mobility Group 4 (HMG) Transcription Factor, High Mobility Group Protein 2a, HMG2a,

Hmgb3 belongs to the high mobility group HMG) protein superfamily. It contains DNA-binding HMG box domains and is thought to play a fundamental role in DNA replication, nucleosome assembly and transcription. Hmgb3 is a sequence-independent chromatin-binding protein, that is preferentially expressed in hematopoietic stem cells. It can alter DNA structure to facilitate transcription factor binding. Enforced expression of Hmgb3 inhibits B-cell and, myeloid differentiation and thus, down-regulation of Hmgb3 protein levels has been concluded to be an important step for myeloid and B-cell differentiation. Hmgb3 deficiency, possibly leads to a failure of hematopoietic stem cells to expand into normal numbers of common lymphoid progenitors and common myeloid progenitors. Hmgb3 progenitors attain the ability to expand rapidly and differentiate into normal numbers of hematopoietic cells. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:64000, Western Blot: 0.01-0.03ug/ml, Optimal dilutions to be determined by the researcher. Positive Control: Human Lung lysates, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.