Anti-Histone H3K9Me2/K14Ac Monoclonal Antibody (RM322)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression, whereas acetylation of histone H3 at lysine 14 (H3K14Ac) is associated with transcriptional activation. H3K9Me2/K14Ac levels are increased in mouse differentiated embryonic stem cells (ESCs) compared with undifferentiated ESCs. Altered H3K9Me2/K14Ac levels have been found in tumor biopsies from patients with HER2+, triple-negative, luminal A-like, or luminal B-like breast cancer. Cayman's Histone H3K9Me2/K14Ac Monoclonal Antibody (RM322) recognizes histone H3 only when modified by both dimethylation at K9 and acetylation at K14. This antibody can be used for multiplex-based assay and Western blot (WB) applications.Immunogen: Peptide corresponding to H3K9Me2/K14Ac. Clone Designation: RM322. Formulation: (Request formulation change), 100 µg of Protein A affinity-purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: Multiplex-based assays and WB. Cross Reactivity: (+) H3K9Me2/K14Ac, (-) Unmodified histone H3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3K9Ac, (-) H3K14Ac, (-) H3K14Me2.