Anti-Histone H3K9Me2 (Clone RM151)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression. Heterochromatin protein 1-alpha (HP1-alpha), HP1-beta, and HP1-gamma selectively bind histone H3 N-terminal peptides containing dimethylated lysine 9 over unmodified lysine 9. High nuclear levels of H3K9Me2 in tumor tissue are associated with decreased disease-specific and disease-free survival in patients with oral and oropharyngeal squamous cell carcinoma. Cayman's Histone H3K9Me2 Monoclonal Antibody (Clone RM151) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Dimethylated Histone H3 Lysine 9. Immunogen: Peptide corresponding to H3K9Me2. Clone Designation: RM151. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS, with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) H3K9Me2, (-) Unmodified histone H3 (1-19), (-) H3K9Me1, (-) H3K9Me3, (-) H3K4Me1, (-) H3K4Me2, (-) H3K4Me3, (-) H3K14Me2, (-) H3K18Me1, (-) H3K18Me2, (-) H3K23Me1, (-) H3K23Me2, (-) H3K27Me1, (-) H3K27Me2, (-) H3K27Me3, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H3K56Me1, (-) H3K79Me1, (-) H3K79Me2, (-) H3K79Me3.