Anti-Histone H3K9Me2

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Dimethylation of histone H3 at lysine 9 (H3K9Me2) is associated with transcriptional repression. Heterochromatin protein 1-alpha (HP1-alpha), HP1-beta, and HP1-gamma selectively bind histone H3 N-terminal peptides containing dimethylated lysine 9 over unmodified lysine 9. High nuclear levels of H3K9Me2 in tumor tissue are associated with decreased disease-specific and disease-free survival in patients with oral and oropharyngeal squamous cell carcinoma. Cayman's Histone H3K9Me2 Polyclonal Antibody can be used for ELISA.Synonyms: Dimethylated Histone H3 Lysine 9. Immunogen: Synthetic peptide from human H3K9Me2 (aa 1-30) conjugated to KLH. Formulation: (Request formulation change), 500 µl of peptide affinity-purified polyclonal antibody. Storage Buffer: TBS, pH 7.4, with 50% glycerol, 0.1% BSA, and 0.02% sodium azide. Host: Rabbit. Applications: ELISA. Cross Reactivity: (+) H3K36Me2 (~50%), (-) Unmodified histone H3, (-) H3K9Me1, (-) H3K9Me3. UniProt Accession #: P68431.