Anti-Histone H3K79Me1 Monoclonal Antibody (RM147)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Methylation of lysine 79 on histone H3 is associated with active chromatin and the silencing of telomere-associated genes by silent information regulator (Sir) proteins. The conversion of H3K79Me1 to H3K79Me2/3 is associated with increased expression of mixed lineage leukemia (MLL) target genes, including Hoxa9. Cayman's Histone H3K79Me1 Monoclonal Antibody (RM147) can be used for chromatin immunoprecipitation (ChIP), ELISA, multiplex-based assays, and Western blot (WB) applications.Synonyms: Monomethylated Histone H3 Lysine 79. Immunogen: Peptide corresponding to H3K79Me1. Clone Designation: RM147. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, multiplex-based assays, WB. Cross Reactivity: (+) H3K79Me1, (-) Unmodified H3K79, (-) H3K79Me2, (-) H3K79Me3, (-) H3K4Me1, (-) H3K4Me2, (-) H3K4Me3, (-) H3K9Me1, (-) H3K9Me2, (-) H3K9Me3, (-) H3K18Me1, (-) H3K18Me2, (-) H3K23Me1, (-) H3K23Me2, (-) H3K27Me1, (-) H3K27Me2, (-) H3K27Me3, (-) H3K36Me1, (-) H3K36Me2, (-) H3K36Me3, (-) H3K56Me1.