Anti-Histone H3K4 Monoclonal Antibody (RM186)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Histone H3 lysine 4 (H3K4) is subject to acetylation and mono-, di-, and trimethylation, and these modifications are found in euchromatic promoter regions and associated with active transcription. Cayman's Histone H3K4 Monoclonal Antibody (RM186) can be used for ELISA, immunocytochemistry (ICC), multiplex-based assays, and Western blot (WB) applications.Synonyms: Unmodified Histone H3 Lysine 4. Immunogen: Peptide corresponding to the N-terminus of histone H3. Clone Designation: RM186. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ELISA, ICC, multiplex-based assays, WB. Cross Reactivity: (+) Unmodified histone H3 (1-9), (+) H3R2Me1 (1-9), (+) H3R2Me2 (1-9), (+) H3T3Ph (1-9), (-) H3K4Ac (1-9), (-) H3K4Me1 (1-9), (-) H3K4Me2 (1-9), (-) H3K4Me3 (1-9).