Anti-Histone H3K18Ac Monoclonal Antibody (RM166)

Histone H3 is a nuclear protein and a component of the nucleosome core, a basic unit of chromatin, that is essential for organizing genomic DNA in eukaryotic nuclei. It is a globular protein that contains an unstructured N-terminal tail that extends outside of the nucleosome core and is subject to various post-translational modifications (PTMs), including methylation, phosphorylation, acetylation, and citrullination. Acetylation of histone H3 at lysine 18 (H3K18Ac) by histone acetyltransferases (HATs) is associated with active gene transcription, whereas deacetylation of H3K18Ac by histone deacetylases (HDACs), including SIRT7, induces transcriptional repression. Tumor H3K18Ac hypoacetylation is associated with poor prognosis and increased risk of tumor recurrence in patients with lung adenocarcinoma or kidney clear cell carcinoma. Cayman's Histone H3K18Ac Monoclonal Antibody (RM166) can be used for chromatin immunoprecipitation (ChIP), ELISA, immunocytochemistry (ICC), immunohistochemistry (IHC), multiplex-based assay, and Western blot (WB) applications.Synonyms: Acetylated Histone H3 Lysine 18. Immunogen: Peptide corresponding to H3K18Ac. Clone Designation: RM166. Formulation: (Request formulation change), 100 µg of protein A-affinity purified monoclonal antibody. Storage Buffer: PBS with 50% glycerol, 1% BSA, and 0.09% sodium azide. Host: Rabbit. Isotype: IgG. Applications: ChIP, ELISA, ICC, IHC, multiplex-based assays, WB. Cross Reactivity: (+) H3K18Ac, (-) H3K18, (-) H3K4Ac, (-) H3K9Ac, (-) H3K14Ac, (-) H3K23Ac, (-) H3K27Ac, (-) H3K36Ac, (-) H3K56Ac, (-) H3K79Ac, (-) H3K122Ac.