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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
H5110-09U.20 | 20 µl | - | - |
3 - 19 business days* |
399.00€
|
||
H5110-09U.100 | 100 µl | - | - |
3 - 19 business days* |
756.00€
|
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You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary... more
Product information "Anti-Histone H2B, ubiquitylated (Lys120) (Histone H2B Type 1-B, Histone H2B.f, H2B/f, H2BFF, Histone"
The nucleosome, made up of four core histone proteins (H2A, H2B, H3 and H4), is the primary building block of chromatin. Originally thought to function as a static scaffold for DNA packaging, histones have now been shown to be dynamic proteins, undergoing multiple types of post-translational modifications, including acetylation, phosphorylation, methylation and ubiquitylation (1). Ubiquitin is a conserved 76aa peptide unit that can be covalently linked to many cellular proteins by the ubiquitylation process. Three components are involved in this protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1, the activated ubiquitin is subsequently transferred to the ubiquitin- carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (2). Histone H2B is mono-ubiquitylated on lysine 120 during transcriptional activation by the RAD6 E2 protein in conjunction with the BRE1A/BRE1B E3 ligase (also known as RNF20/RNF40) (3). The RAD6/BRE1 complex is recruited to gene promoters during activation by the PAF complex, an RNA polymerase II-associated protein complex that regulates transcriptional elongation (3-5). Mono-ubiquitylated histone H2B lysine 120 is associated with the transcribed region of active genes (3,6). Mono-ubiquitylation of histone H2B stimulates transcriptional elongation by facilitating FACT-dependent chromatin remodeling (7,8). In addition, it is essential for subsequent methylation of histone H3 lysines 4 and 79, two additional histone modifications that regulate transcriptional initiation and elongation (9). Interestingly, deubiquitylation of histone H2B lysine 120 by USP22, a subunit of the human SAGA histone acetyltransferase complex, is a required step in transcriptional activation (10). Thus, it appears that the ubiquitylation state of histone H2B is dynamic during transcription and may serve as an intermediate step in transcriptional activation., Applications: , Suitable for use in Immunofluorescence, Flow Cytometry, Western Blot and ChIP. Other applications not tested. Recommended Dilution: Western Blot: 1:1000, incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking, overnight., ChIP: 1:200, Immunofluorescence (IF-IC): 1:1600, Flow Cytometry: 1:100, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Keywords: | Anti-Histone H2B type 1-B |
Supplier: | United States Biological |
Supplier-Nr: | H5110-09U |
Properties
Application: | WB |
Antibody Type: | Monoclonal |
Clone: | 11C1038(D11) |
Conjugate: | No |
Host: | Rabbit |
Species reactivity: | human, monkey, mouse, rat |
Immunogen: | Synthetic peptide corresponding to the carboxy terminus of the human histone H2B protein in which Lys120 is mono-ubiquitylated. |
Format: | Purified |
Database Information
KEGG ID : | K11252 | Matching products |
UniProt ID : | P33778 | Matching products |
Gene ID | GeneID 3018 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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