Anti-Histone H1

The histone proteins serve as a structural scaffold for the organization of nuclear DNA into chromatin. In eukaryotic cells there are 4 core histone proteins, H2A, H2B, H3 and H4 that assemble to form an octamer round which the DNA is wrapped in a sequence dependent manner. The resulting complex is known as a nucleosome and is the basic subunit of chromatin. Histone H1 acts as a linker and binds to each nucleosome, and by self affinity, links these nucleosomes together resulting in the 30nm chromatin fiber. Applications: Suitable for use in ELISA, Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilutions: Immunofluorescence: Speckled staining is observed in the nucleus of normal and malignant cells., Western Blot: 1:100. In blots of rat brain homogenate a duplet is observed at 27-29kD consistent with Histone H1. , Immunohistochemistry (Frozen, paraffin): Acetone or PFA fixation of frozen sections is recommended., Optimal dilutions to be determined by the researcher. Immunohistochemistry Positive Control: Tonsil, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.