Anti-Hepatocyte Growth Factor Receptor (HGF R, c-MET, HGFAC, HGF activator Hepatocyte growth factor

Human hepatocyte growth factor receptor, (HGF R), a product of the proto-oncogene c-MET, is a heterodimeric transmembrane glycoprotein that is a receptor-type tyrosine kinase. HGF R is synthesized as a 1390 aa single-chain precursor which undergoes cotranslational glycosylation and proteolytic cleavage to give rise to the heterodimeric mature form. The 190kD HGF R heterodimer is composed of a 50kD alpha chain (aa 25-307) disulfide-linked to a 145kD beta chain (aa 308-1390). The alpha chain is exposed to the cell surface and the beta chain spans the plasma membrane. Applications: Suitable for use in ELISA. Other applications not tested. Recommended Dilution: ELISA: 2-8ug/ml. Used as a capture reagent in a human HGF R sandwich immunoassay in combination with biotinylated, human HGF R detection antibody and recombinant human HGF R, as the standard., Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute by adding sterile PBS, aliquot and store at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.