Anti-Hemoglobin

Hemoglobin is composed of one globin plus four hemes, heme consists of protoporphyrin IX and ferrous iron. The iron content of human hemoglobin is 0.338% (w/w) which gives a minimal molecular weight of 16,520, however, ultracentrifugal and osmotic pressure measurements indicate an actual molecular weight four times the minimum molecular weight. , Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilutions: Capture ELISA: 5ug/ml , Western Blot: 1:1000-1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.