Anti-Heat Shock Protein 90, beta (Heat Shock Protein HSP 90-beta, HSP90b, HSP 90, D6S182, FLJ26984,

The 90-kDa heat shock protein (Hsp90) is a highly conserved, and abundant cytosolic homodimeric molecular chaperone. Hsp90 is distinguished from other chaperones in that most of its known substrates are signal transduction proteins, non activated steroid hormone receptors, several protooncogenic tyrosine and serine/threonine kinases and actin. Two isoforms which correspond to the major and minor isoform, (Hsp90 alpha and Hsp90 beta) can be found in nearly equal amount in humans, and operates as part of a multichaperone machinery in the cytosol, which includes Hsp70, peptidyl-prolyl isomerases and other co-chaperones. Applications: Suitable for use in Immunofluorescence, Western Blot, and Immunohistochemistry. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:100-1:200, Western Blot: 1:500-1:1,000 , Immunohistochemistry: 1:50-1:100 , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.