Anti-Heat Shock Protein 21 (HSP 21, 25.3kD heat shock protein, chloroplastic, At4g27670, AtHsp25.3,

HSP21 is a cholorplast-localized small heat shock protein. How these small Hsps protect living cells is not fully understood. They seem to be involved in chaperone functions by protecting other proteins from irreversible denaturation. Small Hsps are also produced late in the seed, maturation process. HSP21 has also been shown to protect Photsystem II from temperature-dependent oxidative stress as well as having a role in fruit reddening and the conversion of chloroplasts to chromoplasts (1). Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot:1:3000 using ECL, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.