Anti-Guanine Nucleotide Dissociation Inhibitor (GDI)

Small molecular weight GTP-binding proteins of the Rab family have been shown to play an essential role in the regulation of vesicular trafficking. The cycling of these proteins between the inactive (GDP-bound) and active (GTP-bound) forms is thought to catalyze docking to specific target membranes. To date, several accessory proteins which serve to regulate the association of Rab proteins with GTP or GDP have been identified(4,5,6). The best characterized of these Rab accessory proteins is Rab3A GDI(4,5,7,8). Rab3A GDI forms cytosolic complexes with Rab3A and, in vitro, promotes the dissociation of Rab3A from membranes(7,8). Two other isoforms of GDI have recently been isolated and have been termed GDI b and GDI-2, respectively(3,5). Studies have revealed that the proportion of Rab proteins found associated with a particular GDI isoform depends upon the abundance of the specific GDI in the cytosol, as well as on the tissue and cell type examined(5,6,7). Applications: Suitable for use in ELISA, Western Blotting, Immunoprecipitation. Other applications not tested. Recommended Dilution: ELISA: 0.5-1ug/ml , Immunoprecipitation: 5-10ug , Western Blotting (2) : 1ug/ml , , Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.