Anti-Grb2 (Growth Factor Receptor-Bound Protein 2, ASH, Abundant Src Homology)

GRB-2 (growth factor receptor-bound protein 2), an adaptor protein involved in signal , transduction, contains a central SH2 domain flanked by two SH3 domains. The SH2 domain , binds to phosphotyrosine residues in RTKs such as PDGF and EGF, non-RTKs such as , Bcr/Abl and FAK, and docking proteins such as FRS-2 and Gab1. The SH3 domains , associate with proline rich motifs on the guanine nucleotide releasing factor, Sos, stimulating , GTP binding to Ras, which in turn activates MAPK and other signaling pathways. GRB-2 , also participates in the endocytosis of EGFR through its recruitment of Cbl. Tyrosine , phosphorylation of GRB-2 SH3 domains reduces binding to Sos and negatively regulates , downstream signaling pathways including Ras, JNK and MAPK. , , Applications:, Western Blot: An antibody concentration of 1.0ug/ml is recommended. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile PBS with 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.