Anti-Granulocyte Colony Stimulating Factor (G-CSF), clone 5K129

Applications:, Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: Useful as a detection antibody in a sandwich ELISA for quantifying human G-CSF protein levels., Western Blot: Useful for Western blot applications. A concentration of 1-5ug/ml (in conjunction with AP-labeled Goat Anti-Rat-Ig) has been found to enable detection of < 100ng/lane of recombinant human G-CSF under reducing conditions., Optimal dilutions to be determined by the researcher. *Recommended Secondary Antibodies: I1904-47H IgG, H&L (HRP) Pab GtxRt, I1904-47N IgG, H&L, X-Adsorbed (HRP) Pab GtxRt, I1904-47T IgG, H&L (HRP) Pab GtxRt, I1904-50T1 IgG, H&L (HRP) Pab GtxRt, I1904-50U1 IgG H&L, X-adsorbed (HRP) Pab GtxRt, I1904-74H IgG, Mouse Ig Adsorbed (HRP) Pab GtxRt, Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.