Anti-Glutathione-S-Transferase pi (GSTpi)

GST's are responsible for the metabolism of a broad range of xenobiotics and carcinogens. The enzyme catalyzes the reaction of glutathione with a wide variety of organic compounds to form thioethers, a reaction that is sometimes a first step in a detoxification process leading to mercapturic acid formation. GSTs play an important role defense against electrophilic chemicals generated by cellular oxidative reactions catalyzed by cytochrome P450 and other oxidases. GSTs are believed to defend against the highly reactive electrophiles that are formed as a result of cellular oxidative processes. Based on amino acid sequence similarities and antibody cross-reactivities, the mammalian cytosolic GSTs are divided into the following classes: alpha, mu, kappa, theta, pi, zeta, omega and sigma. GSTs exists as homodimers of ~25kD. GST A1-1 is mainly found in kidney, intestine, lung, and liver. GST M1-1 is restricted to liver and few other tissues. GST P1-1 has a widespread distribution in most tumor cells and tissues but it is absent in liver. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:100,000. Coat ELISA plates with 50-100ng/well GST pi. , Western Blot: 1:500-1:3000 (ECL) , Note: Actual dilution of antibody will vary depending upon the type of technique used to detect primary antibody such colorimetric or chemiluminescence substrates. The nature and concentration of GST in may also affect antibody concentration., Optimal dilutions to be determined by the researcher. Western Blot Positive Control: G8135-26D, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.