Anti-Glutathione-S-Transferase (GST) (Schistosoma japonicum)

pGEX vectors are frequently utilized in bacterial systems to express polypeptides or entire proteins as fusions with glutathione S-transferase. Using this fusion system, a polypeptide sequence is linked to the C-terminus of the 26 kD GST protein from Schistosoma japonicum. The bacterially expressed GST-fusion proteins are purified by absorption with glutathione-agarose beads. The fusion protein is subsequently eluted from the glutathione agarose with an excess of free glutathione. Antibodies to GST are used to detect pGEX-expressed fusion proteins and as immuno-affinity reagents for purification of recombinant proteins. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA (solid phase = antigen): 0.1-1.0ug/ml, ELISA (solid phase = antibody): 1.0ug/ml, Western Blotting: 1ug/ml, Optimal dilution determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.