Anti-Glial Fibrillary Acidic Protein (GFAP, FLJ45472)

Glial fibrillary acidic protein, or GFAP, a class III intermediate filament. During development of the central nervous system, GFAP distinguishes astrocytes from other glial cells. It is involved in various cellular functions, such as cell structure and movement, cell communication and the functioning of the blood­brain barrier. It also plays a role in mitosis by adjusting the filament network present in the cell. Defects in GFAP are a cause of Alexander disease, a rare disorder of the central nervous system affecting mostly males. It is a progressive leukoencephalopathy resulting in mental and physical retardation, dementia, seizures and early death. Applications:, Suitable for use in Western Blot, Immunohistochemistry and RIA. Other applications have not been tested. , Recommended Dilutions: Western Blot: 1:500-1:10,000, Immunohistochemistry: Frozen and paraffin sections. Requires protein digestion pretreatment of paraffin sections e.g. trypsin or pronase., Optimal dilutions to be determined by the researcher. Positive Control for Immunohistochemistry: Brain, , Storage and Stability:, May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.