Anti-GAPDHS (Glyceraldehyde-3-phosphate Dehydrogenase, Testis-specific, Spermatogenic Cell-specific

GAPDS is a 40-44kD member of the GAPDH family of enzymes. Due to a high incidence of proline residues, it may migrate anomalously at 56kD in SDS-PAGE. GAPDH-2 is expressed only in testis as a homotetramer, where it is found in the cytoplasm bound to the sperm fibrous sheath. Unlike GAPDH that has multiple functions, GAPDH-2 appears to participate only in carbohydrate metabolism, generating energy via glycolysis for sperm motility and fertility. Human GAPDH-2 is 408aa in length. There are two NAD binding sites (Asp106 and Asn388) with a catalytic region between aa223-226. One splice variant shows a 9aa insertion after Ser220. Over aa75-222, human GAPDH-2 shares 84% aa identity with mouse GAPDH-2. Applications: Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested. Recommended Dilution: Western Blot: 1ug/ml, Immunohistochemistry: 5-15ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.