Anti-G9a (EHMT2, euchromatic histone-lysine N-methyltransferase 2 ) (C6H3)

Anti-G9a (EHMT2, euchromatic histone-lysine N-methyltransferase 2 ) (C6H3)
Item number Size Datasheet Manual SDS Delivery time Quantity Price
G1009-02.100 100 µl - -

3 - 19 business days*

514.00€
 
G9a is a member of a family of histone lysine methyltransferases, each of which contains a... more
Product information "Anti-G9a (EHMT2, euchromatic histone-lysine N-methyltransferase 2 ) (C6H3)"
G9a is a member of a family of histone lysine methyltransferases, each of which contains a conserved catalytic SET domain originally identified in Drosophila Su[var]3-9, Enhancer of zeste, and Trithorax proteins (1). Recombinant G9a can mono-, di- and tri-methylate histone H3 on Lys9 and Lys27 in vitro (1,2). However, in vivo G9a forms a complex with GLP, a G9a-related histone methyltransferase, and together these proteins function as the major euchromatic histone H3 Lys9 mono- and di-methyltransferases, creating transcriptionally repressive marks that facilitate gene silencing (3,4). G9a methylates itself on Lys165, a modification that regulates the association of HP1 repressor proteins with the G9a/GLP complex (5). The G9a/GLP complex also contains Wiz, a zinc finger protein that is required for G9a/GLP hetero-dimerization and complex stability (6). Wiz contains two CtBP co-repressor binding sites, which mediate the association of the G9a/GLP with the CtBP co-repressor complex (6). In addition, G9a and GLP are components of other large transcriptional co-repressor complexes, such as those involving E2F6 and CDP/cut (7-9). G9a interacts with DNMT1, and both proteins are required for methylation of DNA and histone H3 (Lys9) at replication foci, providing a functional link between histone H3 Lys9 and CpG methylation during DNA replication (10). G9a activity is critical for meiotic prophase progression, as mutant mice deficient in germ line G9a show a large loss of mature gametes (11). In addition, G9a facilitates increased global levels of di-methyl histone H3 (Lys9) during hypoxic stress and increased G9a expression is associated with hepatocelluar carcinoma (12,13). Applications: Suitable for use in Immunofluorescence and Western Blot. Other applications not tested. Recommended Dilution: Immunofluorescence (IF-IC): 1:50, Western Blot: 1:1000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: United States Biological
Supplier-Nr: G1009-02

Properties

Application: IF, WB
Antibody Type: Monoclonal
Clone: 9j132(C6H3)
Conjugate: No
Host: Rabbit
Reactivity: Human, Monkey, Mouse, Rat
Immunogen: Synthetic peptide (KLH-coupled) corresponding to the carboxy terminus of the human G9a/EHMT2 protein.
Purity: Purified by immunoaffinity chromatography.
Format: Affinity Purified

Database Information

Handling & Safety

Storage: -20°C (-20°C Glycerol)
Shipping: +4°C (International: +4°C)
Caution
Our products are for laboratory research use only: Not for administration to humans!
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