Anti-G Protein Coupled Receptor 77 (GPCR77, GPR77, C5L2, C5a Anaphylatoxin Chemotactic Receptor)

C5L2, also known as GPR77, is a 45kD 7TM receptor that binds the complement proteins , C3a, C4a, and C5a as well as the acylation stimulating peptide (ASP). C5L2 is expressed on , various hematopoietic cells where it promotes complement-mediated inflammation. ASP , binding to C5L2 on adipocytes triggers triglyceride synthesis and glucose uptake and inhibits , lipolysis. Human C5L2 shares 60% aa sequence identity with mouse and rat C5L2. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilution: Flow Cytometry: 25ug/ml, Optimal dilutions to be determined by the researcher. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.