Anti-FOXH1 (Forkhead Box H1, Human Homolog of Xenopus Forkhead Activin Signal Transducer 1)

FoxH1 (also known as FAST-1) is a 39kD, class 2, subfamily H member of the FOX , (forkhead box) family of transcription factors. It is a nuclear protein that binds DNA promoter sites. It does not independently regulate transcription but interacts with SMAD2/4 to activate, and SMAD3/4 to inhibit gene transcription. Human FOXH1 is 365 amino acids (aa) in length. It contains an NLS (aa 22-30), one FKH (fork head) DNA-binding domain (aa 32-128) and a SMAD-interaction region (aa 273-354). Over aa 164-272, human FOXH1 is 55% identical to mouse FoxH1. Applications: Suitable for use in Western blot, Immunocytochemistry and Direct ELISA , , Recommended Dilutions: , Western blot: 0.1-0.2ug/ml. The detection limit is ~5 ng/lane under non-reducing and reducing conditions. , Immunocytochemistr: 10ug/ml to detect human FoxH1 in human BG0IV stem cells. Cells were fixed with PBS containing 4% paraformaldehyde for 20 minutes at RT and blocked with PBS containing 10% normal donkey serum, 0.1% Triton X-100, and 1% BSA for 45 minutes at RT. After blocking, cells were incubated with diluted primary antibody for 3 hours at RT followed by donkey anti-sheep lgG at room temperature for 1 hour. Between each step, cells were washed with PBS containing 0.1% BSA. , Direct ELISA: 0.5-1.0ug/ml. The detection limit is~ 0.2 ng/well. , Optimal dilutions should be determined by each laboratory for each application. Hybridoma: Produced in sheep immunized with purified, E. coli-derived, recombinant human FoxH1 , (rhFoxH1, aa 164-272, Accession # O75593). Storage: Lyophilized samples are stable for twelve months from date of receipt when stored , at -20 to -70° C. Upon reconstitution, the antibody can be stored at 2-8° C for 1 month without detectable loss of activity. Reconstituted antibody can also be aliquotted and stored frozen at -20 to -70°C in a manual defrost freezer for six months without detectable loss of activity. Avoid repeated freeze-thaw cycles.