Anti-Fibrillarin, clone 38F3

Anti-Fibrillarin Antibody is directed against yeast Fibrillarin/Nop1p protein. Fibrillarin antibodies are a total IgG fraction from concentrated clarified cell culture supernate. Reactivity is expected against human and rat. Cross-reactivity toward other sources has not be determined. Anti-Fibrillarin antibody is becoming widely used as a convenient marker for nucleoli in a wide variety of species. Protein function: S-adenosyl-L-methionine-dependent methyltransferase that has the ability to methylate both RNAs and proteins. Involved in pre-rRNA processing by catalyzing the site-specific 2'-hydroxyl methylation of ribose moieties in pre-ribosomal RNA (PubMed:1825809). Site specificity is provided by a guide RNA that base pairs with the substrate. Methylation occurs at a characteristic distance from the sequence involved in base pairing with the guide RNA. Involved in the biogenesis of the 18S rRNA. Also acts as a protein methyltransferase by mediating methylation of 'Gln-105' of histone H2A (H2AQ105me), a modification that impairs binding of the FACT complex and is specifically present at 35S ribosomal DNA locus (PubMed:24352239). [The UniProt Consortium]