Anti-FGF-6 (Fibroblast Growth Factor 6)

Applications: , Suitable for use in Neutralization, Western Blot and ELISA. Other applications not tested. Recommended Dilution: Neutralization: ND50 is ~0.025-0.075ug/ml in the presence of 1ng/ml of rhFGF6 and 1ug/ml of heparin, using the FGF6 responsive NR6R-3T3 fibroblasts as target cells. The Neutralization Dose50 (ND50) for this antibody is defined as that concentration of antibody required to yield one-half maximal inhibition of the cytokine activity on a responsive cell line, when that cytokine is present at a concentration just high enough to elicit a maximum response. Western Blot: 0.1-0.2ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is approximately 2ng/lane under non-reducing and reducing conditions. , ELISA: 0.5-1ug/ml with the appropriate secondary reagents to detect human FGF6. The detection limit for rhFGF6 is ~0.16ng/well. Optimal dilutions to be determined by the researcher. , Storage and Stability:, May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.