Anti-FGF-2 basic, neutralizing, (Fibroblast Growth Factor 2b), clone 0.T.50

Basic fibroblast growth factor (bFGF or FGF2) is a member of the fibroblast growth factor family. In normal tissue, bFGF is present in basement membranes and in the subendothelial extracellular matrix of blood vessels. It stays membrane-bound as long as there is no signal peptide. It has been hypothesized that, during both wound healing of normal tissues and tumor development, the action of heparan sulfate-degrading enzymes activates bFGF, thus mediating the formation of new blood vessels. Additionally, bFGF is a critical component of human embryonic stem cell culture medium. Applications: Suitable for use in Neutralization. Not suitable for Western Blot, use F4210-21 Fibroblast Growth Factor 2, Basic (FGF2b). Other applications have not been tested. Recommended Dilutions: Neutralization of bFGF Activity Assay: 2.5ug/ml neutralizes >40% growth of bovine FBHE cells simulated with 5ng/ml of FGF-2/bFGF. , Optimal dilutions to be determined by the researcher. Hybridoma: P3X63-Ag8.U1 myeloma cells with spleen cells from Balb/c mice. Recommended Positive Control: Fetal liver or kidney tissue, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.