Anti-FceRI, alpha (Fc epsilon RI alpha, FCE1A, FCER1A, High Affinity Immunoglobulin epsilon Receptor

High affinity Fc receptor for IgE (Fc epsilon R1) plays a central role in the IgE-mediated allergic response. Fc epsilon R1 consists of four subunits, a high affinity IgE binding alpha subunit, a beta chain and two di-sulphide linked gamma subunits. Clone 9E1 specifically recognises the extracellular D1 domain of the Fc epsilon R1 alpha chain., Fc epsilon R1 is primarily expressed on mast cells and basophils but expression of Fc epsilon R1 has also been reported on monocytes, Langerhans cells and dendritic cells from patients with atopic diseases. Applications: Suitable for use in ELISA, Flow Cytometry, Immunofluorescence, Immunoprecipitation and Western Blot. Other applications not tested. Recommended Dilution: Flow Cytometry: 1:50-1:100, 10ul labels 10e6 cells in 100ul, Optimal dilutions to be determined by the researcher. Recommended Negative Control: I1904-84Q3: IgG2b Murine Negative Control, Hybridoma: Sp2/0-Ag14 myeloma cells with spleen cells from Balb/c mice. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.