Cookie preferences
This website uses cookies, which are necessary for the technical operation of the website and are always set. Other cookies, which increase the comfort when using this website, are used for direct advertising or to facilitate interaction with other websites and social networks, are only set with your consent.
Configuration
Technically required
These cookies are necessary for the basic functions of the shop.
"Allow all cookies" cookie
"Decline all cookies" cookie
CSRF token
Cookie preferences
Currency change
Customer-specific caching
FACT-Finder tracking
Individual prices
Selected shop
Session
Comfort functions
These cookies are used to make the shopping experience even more appealing, for example for the recognition of the visitor.
Note
Show the facebook fanpage in the right blod sidebar
Statistics & Tracking
Affiliate program
Conversion and usertracking via Google Tag Manager
Track device being used
Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
031356.100 | 100 µl | - | - |
3 - 19 business days* |
568.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate... more
Product information "Anti-Enolase 1 (ENO1, 2-phospho-D-glycerate Hydrolyase, alpha Enolase, C-myc Promoter-binding Protei"
Enolases are enzymes which catalyze the conversion of 2-phosphoglycerate to phosphoenolpyruvate in the glycolytic pathway, and also the reverse reaction in gluconeogenesis. The Enzyme Commission classification number for this class of enzyme is EC4.2.1.11, and they can also be refered to as 2-phospho-D-glycerate hydrolases. There are three enolase proteins each coded for by a distinct gene. They are known as enolase 1, 2 and 3 or alternately as alpha, beta and gamma-enolase. Confusingly, although enolase 1 is alpha-enolase, enolase 2 corresponds to gamma-enolase while enolase 3 is also known as beta-enolase. All three enzymes exist as dimers in vivo and the three molecules are very similar in primary amino acids sequence, as can be seen from a sequence alignment downloadable from here. This similarity allows not only homodimers but also most possible heterodimers to form. The expression pattern of the three enzymes differs in a tissue specific manner, so that antibodies to them are useful cell type specific markers, especially as all three molecules are very abundant. Enolase 1 or alpha is also known as non-neuronal enolase (NNE) and is expressed in most kinds of tissue, but is absent from neurons. Abnormal expression of NNE is associated with tumor progression in some breast and head and neck cancer (1, 2). Enolase 2 or gamma is also known as neuron specific enolase (NSE), and we also market antibodies directed against this protein, specifically 031410. A switch from NNE to NSE occurs in the development of neurons (3). Enolase 3 or beta is expressed primarily in muscle cells. Monoclonal antibody 031356 was raised against the N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method (5). This produces a dendrimer presenting 8 peptides to the immune system. The characterization of the antibody has been published in peer reviewed form and in this publication is there referred to as Enol2-53 (4). The antibody was tested for binding to expressed bovine enolase 1, 2 and 3 and shown to be specific for only enolase 1. The HGNC name for this protein is ENO1. Applications: Suitable for use in Immunofluorescence and Western Blot. The NNE protein runs at about ~47kD on SDS-PAGE gels. Other applications not tested. Recommended Dilution: Immunofluorescence: 1:500-1:1000, Western Blot: 1:5000, Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | 031356 |
Properties
Application: | IF, WB |
Antibody Type: | Monoclonal |
Clone: | 13B815 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | bovine, human, mouse, rat, xenopus |
Immunogen: | N-terminal 12 amino acids of bovine enolase 1 which was synthesized on a 8-amine lysine core using the multiple antigen presentation method. This produces a dendrimer presenting 8 peptides to the immune system. |
Purity: | Purified by Protein G affinity chromatography. |
Format: | Affinity Purified |
Database Information
KEGG ID : | K01689 | Matching products |
UniProt ID : | Q9XSJ4 | Matching products |
Gene ID | GeneID 281141 | Matching products |
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
more
You will get a certificate here
Viewed