Anti-Endonuclease III, the product of nth gene (hNTH1)

Reactive oxygen species, generated during normal cellular activities or by ionizing radiation and by many oxidizing/alkylating agents, can modify (damage) DNA bases by disrupting the phosphodiester backbone. Unrepaired damaged/modified DNA has been implicated in aging and cancer. Many proteins that are involved in BER (base excision repair) have been identified in E. coli. Human homolog of E. coli Endonuclease III, the product of the nth gene (hNTH1), catalyzes the excision of pyrimidines damaged by ring opening or ring saturation. hNTH1 also possesses an associated lyase activity that incises the DNA backbone adjacent to apurinic/apyrimidinic sites. hNTH1 gene (chromosome 16p13.3 encodes a protein of 312aa. Applications: Suitable for use in ELISA. Western Blot, though not tested, may potentially be used as an application. Other applications not tested. Recommended Dilution: ELISA: 1:10,000-1:50,000. Use 50-100ng control antigen/well., Western Blot: 1:1000-1:2000, Detects ~34kD band from human cells and tissues., Optimal dilutions to be determined by the researcher. Control Antigen: N6000-29: Endonuclease III, Human (Product of nth Gene, hNTH1), Storage and Stability: For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.