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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E3375-16.50 | 50 µg | - | - |
3 - 19 business days* |
568.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
Applications:|Suitable for use in Western Blot, Immunoprecipitation. Other applications not... more
Product information "Anti-EGF-Receptor (Epidermal Growth Factor Recptor, erbB, EGFR) (nonPhospho-Y1173), clone 0.T.197"
Applications:, Suitable for use in Western Blot, Immunoprecipitation. Other applications not tested. Recommended Dilution: Western Blot: 0.5-2ug/ml detects the EGF receptor (unphosphorylated at Y1173) in RIPA lysates from unstimulated HFF cells. Lysates from EGF stimulated and unstimulated HFF cells, 10ug per lane, were resolved by electrophoresis and transferred to nitro-cellulose. Lanes 1 and 2 were probed with anti-EGF receptor and visualized using a rabbit anti-sheep secondary antibody conjugated to HRP. Lanes 3 and 4 were probed with anti-EGF-R (non-phospho-Y1173) (1ug/ml) and visualized using a goat anti-mouse secondary antibody conjugated to HRP. A chemiluminescence detection system was used. (EGF-R 175 kD). , Immunoprecipitation: 1-10ug of this antibody is reported to immunoprecipitate the EGF receptor (unphosphorylated at Y1173) from 10e6 vanadate treated A431 cells. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | E3375-16 |
Properties
Application: | IP, WB |
Antibody Type: | Monoclonal |
Clone: | 0.T.197 |
Conjugate: | No |
Host: | Mouse |
Species reactivity: | human, mouse |
Format: | Highly Purified |
Database Information
Handling & Safety
Storage: | -20°C |
Shipping: | +4°C (International: -20°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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