Anti-EGF-Receptor (Epidermal Growth Factor Recptor, erbB, EGFR), clone 3H2090

EGFR is type I receptor tyrosine kinase with sequence homology to erbB-1, -2, -3 -4 or HER-1, -2,-3 -4. It binds to Epidermal Growth Factor (EGF), Transforming Growth Factor-a (TGF-a), Heparin-binding EGF (HB-EGF), amphiregulin, betacellulin and epiregulin. EGFR is overexpressed in tumors of breast, brain, bladder, lung, gastric, head and neck, esophagus, cervix, vulva, ovary, and endometrium. It is predominantly present in squamous cell carcinomas. Applications: Suitable for use in Flow Cytometry, Immunoprecipitation and Immunohistochemistry. Not suitable for use in Western Blot. Other applications not tested. Recommended Dilutions: Flow Cytometry: 1:50-1:100. Use 10ul to label 10e6 cells in 100ul., Immunohistochemistry: Frozen and paraffin sections , Optimal dilutions to be determined by the researcher. Recommended Secondary Reagent: I1904-49H: IgG, F(ab')2, H&L X-Adsorbed (HRP) RbxRt, I1904-47H: IgG, H&L (HRP) Pab GtxRt, Positive Control: Breast carcinoma, Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.