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Item number | Size | Datasheet | Manual | SDS | Delivery time | Quantity | Price |
---|---|---|---|---|---|---|---|
E2208-01E.100 | 100 tests | - | - |
3 - 19 business days* |
871.00€
|
If you have any questions, please use our Contact Form.
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
You can also order by e-mail: info@biomol.com
Larger quantity required? Request bulk
EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G... more
Product information "Anti-EDG1 (Endothelial Cell Differentiation Gene 1) (PE)"
EDG1 is a member of the endothelial differentiation gene family. It is a high affinity G protein-coupled receptor for the bioactive lipid, Sphingosine-1-phosphate. EDG-1 signaling regulates endothelial cell survival, cytoskeletal remodeling, chemotaxis and angiogenesis. Applications: Suitable for use in Flow Cytometry. Other applications not tested. Recommended Dilutions: Flow Cytometry: 10ul labels 10e6 cells. Validated using CHO cell line transfected with EDG1, Optimal dilutions to be determined by the researcher. Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of human IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction., 2. Add 10ul of E2208-01E to <1x10e6 cells and incubate for 30 minutes at RT., 3. Removed unbound antibody by washing the cells twice with Flow Cytometry Staining Buffer (F4568-10). Note that whole blood requires a RBC lysis step at this point. , 4. Resuspend the cells in F4568-10 for analysis. As a control, cells in a separate tube should be treated with PE-labeled mouse IgG2b antibody. Storage and Stability: Store product at 4°C in the dark. DO NOT FREEZE! Stable at 4°C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Supplier: | United States Biological |
Supplier-Nr: | E2208-01E |
Properties
Application: | FC |
Antibody Type: | Monoclonal |
Clone: | 8H120 |
Conjugate: | Phycoerythrin |
Host: | Mouse |
Species reactivity: | human |
Immunogen: | NS0 cells transfected with hEDG-1 |
Format: | Affinity Purified |
Database Information
KEGG ID : | K04288 | Matching products |
UniProt ID : | P21453 | Matching products |
Gene ID | GeneID 1901 | Matching products |
Handling & Safety
Storage: | +4°C |
Shipping: | +4°C (International: +4°C) |
Caution
Our products are for laboratory research use only: Not for administration to humans!
Our products are for laboratory research use only: Not for administration to humans!
Information about the product reference will follow.
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